| 1 | Marianne Vos | in 22h06'11" | Olanda | Rabobank Women Team [Donne] |
| 2 | Evelyn Stevens | a 2'01" | Usa | Team Specialized - Lululemon [Donne] |
| 3 | Emma Pooley | a 3'23" | Gran Bretagna | AA Drink - Leontien.nl Cycling Team [Donne] |
| 4 | Fabiana Luperini | a 3'56" | Italia | Faren Honda Team [Donne] |
| 5 | Judith Arndt | a 4'47" | Germania | Orica - AIS [Donne] |
| 6 | Tatiana Guderzo | a 5'17" | Italia | MCipollini Giambenini [Donne] |
| 7 | Claudia Häusler Lichtenberg | a 5'30" | Germania | Orica - AIS [Donne] |
| 8 | Elisa Longo Borghini | a 6'24" | Italia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 9 | Ashleigh Moolman-Pasio | a 6'29" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 10 | Tiffany Cromwell | a 6'31" | Australia | Orica - AIS [Donne] |
| 11 | Emma Johansson | a 7'54" | Svezia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 12 | Alena Amialiusik | a 11'35" | Bielorussia | Be Pink [Donne] |
| 13 | Rasa Leleivyte | a 13'15" | Lituania | Vaiano Tepso [Donne] |
| 14 | Joanna Van de Winkel | a 18'45" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 15 | Rossella Ratto | a 19'36" | Italia | Verinlegno - Fabiani [Donne] |
| 16 | Shara Gillow | a 19'57" | Australia | Orica - AIS [Donne] |
| 17 | Sharon Laws | a 22'29" | Gran Bretagna | AA Drink - Leontien.nl Cycling Team [Donne] |
| 18 | Katazyna Sosna | a 23'14" | Lituania | Vaiano Tepso [Donne] |
| 19 | Amanda Spratt | a 23'59" | Australia | Orica - AIS [Donne] |
| 20 | Noemi Cantele | a 25'52" | Italia | Be Pink [Donne] |
| 21 | Lise Olivier | a 27'00" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 22 | Tatiana Antoshina | a 27'21" | Russia | Rabobank Women Team [Donne] |
| 23 | Linda Melanie Villumsen Serup | a 28'01" | Danimarca | Orica - AIS [Donne] |
| 24 | Valentina Carretta | a 30'59" | Italia | MCipollini Giambenini [Donne] |
| 25 | Alexandra Burchenkova | a 32'53" | Russia | S.C. Michela Fanini Rox [Donne] |
| 26 | Shelley Olds | a 33'36" | Usa | AA Drink - Leontien.nl Cycling Team [Donne] |
| 27 | Romy Kasper | a 34'38" | Germania | RusVelo [Donne] |
| 28 | Lucinda Brand | a 35'06" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 29 | Inga Cilvinaite | a 35'55" | Lituania | Diadora - Pasta Zara [Donne] |
| 30 | Anna Van der Breggen | a 36'34" | Olanda | Sengers Ladies Cycling Team [Donne] |
