| 31 | Oxana Kozonchuk | a 38'25" | Russia | Be Pink [Donne] |
| 32 | Elke Gebhardt | a 44'01" | Germania | Be Pink [Donne] |
| 33 | Marta Tagliaferro | a 44'31" | Italia | MCipollini Giambenini [Donne] |
| 34 | Viviana Gatto | a 44'44" | Italia | Top Girls Fassa Bortolo - Servetto [Donne] |
| 35 | Petra Zrimsek | a 45'14" | Slovenia | Be Pink [Donne] |
| 36 | Francesca Cauz | a 46'39" | Italia | Top Girls Fassa Bortolo - Servetto [Donne] |
| 37 | Liesbeth De Vocht | a 49'01" | Belgio | Rabobank Women Team [Donne] |
| 38 | Patricia Schwager | a 50'05" | Svizzera | Team GSD Gestion [Donne] |
| 39 | Adrie Visser | a 50'15" | Olanda | Skil 1T4I [Donne] |
| 40 | Tetyana Riabchenko | a 50'24" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 41 | Loes Gunnewijk | a 51'15" | Olanda | Orica - AIS [Donne] |
| 42 | Verónica Leal Balderas | a 52'04" | Messico | S.C. Michela Fanini Rox [Donne] |
| 43 | Charlotte Becker | a 53'21" | Germania | Team Specialized - Lululemon [Donne] |
| 44 | Sanne Van Paassen | a 53'30" | Olanda | Rabobank Women Team [Donne] |
| 45 | Chloe Hosking | a 53'43" | Australia | Team Specialized - Lululemon [Donne] |
| 46 | Giorgia Bronzini | a 55'27" | Italia | Diadora - Pasta Zara [Donne] |
| 47 | Susanna Zorzi | a 55'47" | Italia | MCipollini Giambenini [Donne] |
| 48 | Monia Baccaille | a 55'49" | Italia | MCipollini Giambenini [Donne] |
| 49 | Cherise Taylor | a 56'19" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 50 | Martine Bras | a 56'27" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
| 51 | Jessie Daams | a 57'33" | Belgio | AA Drink - Leontien.nl Cycling Team [Donne] |
| 52 | Martina Ruzickova | a 57'35" | Repubblica Ceca | S.C. Michela Fanini Rox [Donne] |
| 53 | Marta Bastianelli | a 57'37" | Italia | MCipollini Giambenini [Donne] |
| 54 | Lise Nöstvold | a 57'48" | Norvegia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 55 | Nicole Cooke | a 58'11" | Gran Bretagna | Faren Honda Team [Donne] |
| 56 | Alessandra Borchi | a 59'13" | Italia | MCipollini Giambenini [Donne] |
| 57 | Katie Colclough | a 1h00'49" | Gran Bretagna | Team Specialized - Lululemon [Donne] |
| 58 | Jennifer Fiori | a 1h01'55" | Italia | Top Girls Fassa Bortolo - Servetto [Donne] |
| 59 | Lisa Brennauer | a 1h03'48" | Germania | Team Specialized - Lululemon [Donne] |
| 60 | Roxane Knetemann | a 1h04'35" | Olanda | Rabobank Women Team [Donne] |
