| 31 | Dalia Muccioli | a 15'23" | Italia | Be Pink [Donne] |
| 32 | Valentina Carretta | a 15'36" | Italia | MCipollini Giordana [Donne] |
| 33 | Pauline Ferrand-Prévot | a 15'43" | Francia | Rabo Women Cycling Team [Donne] |
| 34 | Serika Guluma | a 17'45" | Colombia | Vaiano Fondriest [Donne] |
| 35 | Christel Ferrier-Bruneau | a 17'49" | Francia | Faren - Kuota [Donne] |
| 36 | Lucinda Brand | a 20'06" | Olanda | Rabo Women Cycling Team [Donne] |
| 37 | Elena Kuchinskaya Andreeva | a 21'19" | Russia | RusVelo [Donne] |
| 38 | Valentina Scandolara | a 21'55" | Italia | MCipollini Giordana [Donne] |
| 39 | Aude Biannic | a 23'31" | Francia | S.C. Michela Fanini Rox [Donne] |
| 40 | Andrea Dvorak | a 25'16" | Usa | Exergy Twenty16 [Donne] |
| 41 | Roxane Knetemann | a 25'20" | Olanda | Rabo Women Cycling Team [Donne] |
| 42 | Aizhan Zhaparova | a 25'40" | Russia | RusVelo [Donne] |
| 43 | Amy Pieters | a 26'24" | Olanda | Team Argos - Shimano [Donne] |
| 44 | Lorena Maria Vargas Villamil | a 28'03" | Colombia | Pasta Zara - Cogeas - Manhattan [Donne] |
| 45 | Sari Saarelainen | a 28'26" | Finlandia | MCipollini Giordana [Donne] |
| 46 | Adrie Visser | a 28'39" | Olanda | Boels - Dolmans Cyclingteam [Donne] |
| 47 | Anastasia Chulkova | a 28'43" | Russia | RusVelo [Donne] |
| 48 | Susanna Zorzi | a 29'01" | Italia | Faren - Kuota [Donne] |
| 49 | Joanne Kiesanowski | a 31'39" | Nuova Zelanda | TIBCO - To The Top [Donne] |
| 50 | Lauren Robertson Stephens | a 32'57" | Usa | TIBCO - To The Top [Donne] |
| 51 | Jasmin Glaesser | a 34'10" | Canada | TIBCO - To The Top [Donne] |
| 52 | Inga Cilvinaite | a 34'40" | Lituania | Pasta Zara - Cogeas - Manhattan [Donne] |
| 53 | Romy Kasper | a 35'21" | Germania | Boels - Dolmans Cyclingteam [Donne] |
| 54 | Barbara Guarischi | a 35'26" | Italia | Vaiano Fondriest [Donne] |
| 55 | Alessandra D'Ettorre | a 35'27" | Italia | Vaiano Fondriest [Donne] |
| 56 | Iris Slappendel | a 35'50" | Olanda | Rabo Women Cycling Team [Donne] |
| 57 | Gracie Elvin | a 36'14" | Australia | Orica - AIS [Donne] |
| 58 | Ane Santesteban Gonzalez | a 36'48" | Spagna | Bizkaia - Durango [Donne] |
| 59 | Loren Rowney | a 37'10" | Australia | Specialized - Lululemon [Donne] |
| 60 | Giada Borgato | a 38'58" | Italia | Pasta Zara - Cogeas - Manhattan [Donne] |
