| 46 | Cayetano José Sarmiento Tunarrosa | a 3'04" | Colombia | Cannondale Pro Cycling Team |
| 47 | Stefano Pirazzi | a 3'06" | Italia | Bardiani Valvole - CSF Inox |
| 48 | Fabio Andrés Duarte Arevalo | a 3'06" | Colombia | Colombia |
| 49 | Enrico Battaglin | a 3'07" | Italia | Bardiani Valvole - CSF Inox |
| 50 | Francis Mourey | a 3'09" | Francia | FDJ.fr |
| 51 | Petr Ignatenko | a 3'18" | Russia | Katusha Team |
| 52 | Tanel Kangert | a 3'20" | Estonia | Astana Pro Team |
| 53 | Vladimir Karpets | a 3'36" | Russia | Movistar Team |
| 123 | Daniele Pietropolli | a 30'17" | Italia | Lampre - Merida |
| 124 | Murilo Antonio Fischer | a 30'23" | Brasile | FDJ.fr |
| 125 | Maurits Lammertink | a 30'37" | Olanda | Vacansoleil Pro Cycling Team |
| 126 | Matti Breschel | a 30'51" | Danimarca | Team Saxo - Tinkoff |
| 127 | Johan Le Bon | a 30'52" | Francia | FDJ.fr |
| 128 | Cameron Wurf | a 30'57" | Australia | Cannondale Pro Cycling Team |
| 4 | Vincenzo Nibali | a 31" | Italia | Astana Pro Team |
| 129 | Matthew Harley Goss | a 31'39" | Australia | Orica - GreenEDGE |
| 130 | Daniel Oss | a 31'48" | Italia | BMC Racing Team |
| 131 | Stefano Garzelli | a 31'48" | Italia | Vini Fantini - Selle Italia |
| 132 | Giacomo Nizzolo | a 31'54" | Italia | RadioShack - Leopard |
| 133 | Anthony Roux | a 32'06" | Francia | FDJ.fr |
| 134 | Giairo Ermeti | a 32'14" | Italia | Androni Giocattoli - Venezuela |
| 135 | Luka Mezgec | a 32'24" | Slovenia | Team Argos - Shimano |
| 136 | Dominique Rollin | a 32'25" | Canada | FDJ.fr |
| 137 | Mark Cavendish | a 32'26" | Gran Bretagna | Omega Pharma - Quick Step |
| 138 | Stef Clement | a 32'26" | Olanda | Belkin Pro Cycling Team |
| 139 | Fabio Sabatini | a 32'33" | Italia | Cannondale Pro Cycling Team |
| 140 | Gianluca Brambilla | a 32'40" | Italia | Omega Pharma - Quick Step |
| 141 | Oscar Gatto | a 32'56" | Italia | Vini Fantini - Selle Italia |
| 142 | Dirk Bellemakers | a 32'58" | Olanda | Lotto Belisol Team |
| 143 | Christian Vande Velde | a 33'00" | Usa | Garmin - Sharp |
