| 31 | Sharon Laws | a 1'11" | Gran Bretagna | AA Drink - Leontien.nl Cycling Team [Donne] |
| 32 | Oxana Kozonchuk | a 1'11" | Russia | Be Pink [Donne] |
| 33 | Liesbeth De Vocht | a 1'11" | Belgio | Rabobank Women Team [Donne] |
| 34 | Valentina Carretta | a 1'11" | Italia | MCipollini Giambenini [Donne] |
| 35 | Claudia Häusler Lichtenberg | a 1'11" | Germania | Orica - AIS [Donne] |
| 36 | Rachel Neylan | a 1'11" | Australia | Abus - Nutrixxion [Donne] |
| 37 | Ina-Yoko Teutenberg | a 1'11" | Germania | Team Specialized - Lululemon [Donne] |
| 38 | Amber Neben | a 1'11" | Usa | Team Specialized - Lululemon [Donne] |
| 39 | Romy Kasper | a 1'11" | Germania | RusVelo [Donne] |
| 40 | Natalia Boyarskaya | a 1'21" | Russia | RusVelo [Donne] |
| 41 | Nathalie Lamborelle | a 1'21" | Lussemburgo | Kleo Ladies Team [Donne] |
| 42 | Nina Kessler | a 1'21" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
| 43 | Sara Mustonen | a 1'21" | Svezia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 44 | Polona Batagelj | a 1'21" | Slovenia | Diadora - Pasta Zara [Donne] |
| 45 | Petra Zrimsek | a 1'21" | Slovenia | Be Pink [Donne] |
| 46 | Laura Van Der Kamp | a 1'21" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
| 47 | Alessandra Borchi | a 1'21" | Italia | MCipollini Giambenini [Donne] |
| 48 | Thalita De Jong | a 1'21" | Olanda | Rabobank Women Team [Donne] |
| 49 | Ursa Pintar | a 1'21" | Slovenia | |
| 50 | Mieke Kröger | a 1'21" | Germania | |
| 51 | Annelie Gärtner | a 1'21" | Germania | |
| 52 | Evgenya Vysotska | a 1'21" | Ucraina | |
| 53 | Olena Sharga | a 1'21" | Ucraina | |
| 54 | Esther Fennel | a 1'21" | Germania | |
| 55 | Tetyana Riabchenko | a 1'27" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 56 | Irina Molicheva | a 1'29" | Russia | RusVelo [Donne] |
| 57 | Olena Pavlukhina | a 1'32" | Ucraina | |
| 58 | Kelly Markus | a 1'32" | Olanda | Skil 1T4I [Donne] |
| 59 | Anouska Koster | a 1'35" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
| 60 | Daniela Gass | a 1'49" | Germania | Abus - Nutrixxion [Donne] |
