| 31 | Ursa Pintar | a 2'23" | Slovenia | |
| 32 | Anna-Bianca Schnitzmeier | a 2'24" | Germania | Abus - Nutrixxion [Donne] |
| 33 | Polona Batagelj | a 2'25" | Slovenia | Diadora - Pasta Zara [Donne] |
| 34 | Denise Ramsden | a 2'31" | Canada | |
| 35 | Hanna Nilsson | a 2'31" | Svezia | Alriksson Go:Green [Donne] |
| 36 | Amanda Spratt | a 2'36" | Australia | Orica - AIS [Donne] |
| 37 | Natalia Boyarskaya | a 2'38" | Russia | RusVelo [Donne] |
| 38 | Annelie Gärtner | a 2'42" | Germania | |
| 39 | Jessie MacLean | a 2'57" | Australia | Orica - AIS [Donne] |
| 40 | Simona Frapporti | a 3'09" | Italia | Be Pink [Donne] |
| 41 | Marijn De Vries | a 3'12" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 42 | Sara Mustonen | a 3'12" | Svezia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 43 | Marta Bastianelli | a 3'15" | Italia | MCipollini Giambenini [Donne] |
| 44 | Alexis Rhodes | a 3'16" | Australia | Orica - AIS [Donne] |
| 45 | Julia Martisova | a 3'17" | Russia | Be Pink [Donne] |
| 46 | Sarah-Lena Hofmann | a 3'27" | Germania | |
| 47 | Petra Zrimsek | a 3'35" | Slovenia | Be Pink [Donne] |
| 48 | Maryna Ivaniuk | a 3'42" | Ucraina | |
| 49 | Sabrina Stultiens | a 4'43" | Olanda | Rabobank Women Team [Donne] |
| 50 | Sylwia Kapusta | a 5'11" | Polonia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 51 | Chloe Hosking | a 5'17" | Australia | Team Specialized - Lululemon [Donne] |
| 52 | Liesbeth De Vocht | a 5'18" | Belgio | Rabobank Women Team [Donne] |
| 53 | Laura Van Der Kamp | a 5'19" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
| 54 | Tetyana Riabchenko | a 5'19" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 55 | Isabelle Söderberg | a 5'27" | Svezia | AA Drink - Leontien.nl Cycling Team [Donne] |
| 56 | Alessandra Borchi | a 5'32" | Italia | MCipollini Giambenini [Donne] |
| 57 | Malin Rydlund | a 5'36" | Svezia | Alriksson Go:Green [Donne] |
| 58 | Beate Zanner | a 5'36" | Germania | |
| 59 | Annelies Van Doorslaer | a 6'13" | Belgio | Kleo Ladies Team [Donne] |
| 60 | Sari Saarelainen | a 6'45" | Finlandia | Forno d'Asolo Colavita [Donne] |
