| 31 | Shelley Olds | a 32'43" | Usa | AA Drink - Leontien.nl Cycling Team [Donne] |
| 32 | Verónica Leal Balderas | a 33'28" | Messico | S.C. Michela Fanini Rox [Donne] |
| 33 | Romy Kasper | a 33'45" | Germania | RusVelo [Donne] |
| 34 | Inga Cilvinaite | a 35'02" | Lituania | Diadora - Pasta Zara [Donne] |
| 35 | Anna Van der Breggen | a 35'41" | Olanda | Sengers Ladies Cycling Team [Donne] |
| 36 | Oxana Kozonchuk | a 37'32" | Russia | Be Pink [Donne] |
| 37 | Martina Ruzickova | a 38'59" | Repubblica Ceca | S.C. Michela Fanini Rox [Donne] |
| 38 | Elke Gebhardt | a 43'08" | Germania | Be Pink [Donne] |
| 39 | Alessandra Giuseppina Grassi Herrera | a 43'18" | Messico | Faren Honda Team [Donne] |
| 40 | Marta Tagliaferro | a 43'38" | Italia | MCipollini Giambenini [Donne] |
| 41 | Petra Zrimsek | a 44'21" | Slovenia | Be Pink [Donne] |
| 42 | Robyn De Groot | a 47'05" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 43 | Liesbeth De Vocht | a 48'08" | Belgio | Rabobank Women Team [Donne] |
| 44 | Giada Borgato | a 49'04" | Italia | Diadora - Pasta Zara [Donne] |
| 45 | Adrie Visser | a 49'22" | Olanda | Skil 1T4I [Donne] |
| 46 | Tetyana Riabchenko | a 49'31" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 47 | Loes Gunnewijk | a 50'22" | Olanda | Orica - AIS [Donne] |
| 48 | Ina-Yoko Teutenberg | a 50'37" | Germania | Team Specialized - Lululemon [Donne] |
| 49 | Charlotte Becker | a 52'29" | Germania | Team Specialized - Lululemon [Donne] |
| 50 | Sanne Van Paassen | a 52'37" | Olanda | Rabobank Women Team [Donne] |
| 51 | Jennifer Hohl | a 52'40" | Svizzera | Faren Honda Team [Donne] |
| 52 | Jessie Daams | a 52'40" | Belgio | AA Drink - Leontien.nl Cycling Team [Donne] |
| 53 | Chloe Hosking | a 52'50" | Australia | Team Specialized - Lululemon [Donne] |
| 54 | Malgorzata Jasinska | a 53'11" | Polonia | MCipollini Giambenini [Donne] |
| 55 | Ann Sophie Duyck | a 53'48" | Belgio | Lotto Belisol Ladies [Donne] |
| 56 | Monia Baccaille | a 54'33" | Italia | MCipollini Giambenini [Donne] |
| 57 | Giorgia Bronzini | a 54'34" | Italia | Diadora - Pasta Zara [Donne] |
| 58 | Susanna Zorzi | a 54'54" | Italia | MCipollini Giambenini [Donne] |
| 59 | Cherise Taylor | a 55'26" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 60 | Martine Bras | a 55'34" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
