| 31 | Adrie Visser | a 1'32" | Olanda | Skil 1T4I [Donne] |
| 32 | Carla Ryan | a 1'32" | Australia | AA Drink - Leontien.nl Cycling Team [Donne] |
| 33 | Julie Krasniak | a 1'35" | Francia | |
| 34 | Sylwia Kapusta | a 1'44" | Polonia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 35 | Kristin McGrath | a 1'48" | Usa | Exergy Twenty12 [Donne] |
| 36 | Natalia Boyarskaya | a 1'50" | Russia | RusVelo [Donne] |
| 37 | Larisa Pankova | a 1'52" | Russia | |
| 38 | Claudia Häusler Lichtenberg | a 2'00" | Germania | Orica - AIS [Donne] |
| 39 | Christel Ferrier-Bruneau | a 2'09" | Francia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 40 | Manon Souyris | a 2'55" | Francia | Vienne Futuroscope [Donne] |
| 41 | Tatiana Antoshina | a 3'55" | Russia | Rabobank Women Team [Donne] |
| 42 | Siobhan Dervan Horgan | a 5'12" | Irlanda | Team GSD Gestion [Donne] |
| 43 | Joanna Van de Winkel | a 5'12" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 44 | Valentina Scandolara | a 5'14" | Italia | S.C. Michela Fanini Rox [Donne] |
| 45 | Alexia Muffat | a 5'14" | Francia | ASPTT Dijon - Bourgogne [Donne] |
| 46 | Maaike Polspoel | a 5'14" | Belgio | Topsport Vlaanderen - Ridley Team [Donne] |
| 47 | Annelies Van Doorslaer | a 5'19" | Belgio | Kleo Ladies Team [Donne] |
| 48 | Sofie De Vuyst | a 5'21" | Belgio | Lotto Belisol Ladies [Donne] |
| 49 | Alexandra Burchenkova | a 5'21" | Russia | S.C. Michela Fanini Rox [Donne] |
| 50 | Patricia Schwager | a 5'21" | Svizzera | Team GSD Gestion [Donne] |
| 51 | Ine Beyen | a 5'21" | Belgio | Topsport Vlaanderen - Ridley Team [Donne] |
| 52 | Pauliena Rooijakkers | a 5'27" | Olanda | Dolmans - Boels Cyclingteam [Donne] |
| 53 | Elena Berlato | a 5'29" | Italia | Top Girls Fassa Bortolo - Servetto [Donne] |
| 54 | Olga Zabelinskaya | a 5'29" | Russia | RusVelo [Donne] |
| 55 | Edwige Pitel | a 5'29" | Francia | |
| 56 | Polona Batagelj | a 5'29" | Slovenia | Diadora - Pasta Zara [Donne] |
| 57 | Valentina Carretta | a 5'31" | Italia | MCipollini Giambenini [Donne] |
| 58 | Anna Sanchis Chafer | a 5'32" | Spagna | Bizkaia - Durango [Donne] |
| 59 | Oxana Kozonchuk | a 5'32" | Russia | Be Pink [Donne] |
| 60 | Petra Dijkman | a 5'32" | Olanda | Kleo Ladies Team [Donne] |
