| 4 | Marianne Vos | a 25" | Olanda | Nederland Bloeit [Donne] |
| 5 | Emma Johansson | a 25" | Svezia | Red Sun Cycling Team [Donne] |
| 6 | Emma Pooley | a 25" | Gran Bretagna | Cervélo Test Team [Donne] |
| 7 | Christel Ferrier-Bruneau | a 25" | Francia | Vienne Futuroscope [Donne] |
| 8 | Liesbeth De Vocht | a 25" | Belgio | Nederland Bloeit [Donne] |
| 9 | Regina Bruins | a 25" | Olanda | Cervélo Test Team [Donne] |
| 10 | Trixi Worrack | a 25" | Germania | Noris Cycling [Donne] |
| 11 | Martine Bras | a 25" | Olanda | Gauss RDZ Ormu [Donne] |
| 12 | Chantal Blaak | a 25" | Olanda | Leontien.nl [Donne] |
| 13 | Madeleine Sandig | a 25" | Germania | Noris Cycling [Donne] |
| 14 | Annemiek Van Vleuten | a 25" | Olanda | Nederland Bloeit [Donne] |
| 15 | Claudia Häusler Lichtenberg | a 25" | Germania | Cervélo Test Team [Donne] |
| 16 | Carla Ryan | a 25" | Australia | Cervélo Test Team [Donne] |
| 17 | Sharon Laws | a 25" | Gran Bretagna | Cervélo Test Team [Donne] |
| 18 | Adrie Visser | a 25" | Olanda | HTC - Columbia Women [Donne] |
| 19 | Elizabeth Armitstead | a 25" | Gran Bretagna | Cervélo Test Team [Donne] |
| 20 | Loes Gunnewijk | a 25" | Olanda | Nederland Bloeit [Donne] |
| 26 | Inga Cilvinaite | a 3'00" | Lituania | Safi - Pasta Zara [Donne] |
| 27 | Petra Dijkman | a 3'00" | Olanda | Red Sun Cycling Team [Donne] |
| 28 | Joanne Kiesanowski | a 3'00" | Nuova Zelanda | Team TIBCO [Donne] |
| 29 | Rebecca Much | a 3'00" | Usa | Team TIBCO [Donne] |
| 30 | Emma Mackie | a 3'00" | Australia | Team TIBCO [Donne] |
| 31 | Angela Brodtka Hennig | a 3'00" | Germania | Noris Cycling [Donne] |
| 32 | Vicki Whitelaw Eustace | a 3'00" | Australia | Lotto Ladies Team [Donne] |
| 33 | Martina Zwick | a 3'00" | Germania | |
| 34 | Sylwia Kapusta | a 3'00" | Polonia | Safi - Pasta Zara [Donne] |
| 35 | Marie Lindberg | a 3'00" | Svezia | Red Sun Cycling Team [Donne] |
| 36 | Irene Van den Broek | a 3'00" | Olanda | Leontien.nl [Donne] |
| 37 | Romy Kasper | a 3'00" | Germania | Noris Cycling [Donne] |
| 38 | Josien Van Wingerden | a 3'00" | Olanda | Leontien.nl [Donne] |
