| 1 | Mara Abbott | in 17h14'34" | Usa | Exergy Twenty16 [Donne] |
| 2 | Tatiana Guderzo | a 2'40" | Italia | MCipollini Giordana [Donne] |
| 3 | Claudia Häusler Lichtenberg | a 2'55" | Germania | TIBCO - To The Top [Donne] |
| 4 | Francesca Cauz | a 3'10" | Italia | Top Girls Fassa Bortolo [Donne] |
| 5 | Shara Gillow | a 4'50" | Australia | Orica - AIS [Donne] |
| 6 | Marianne Vos | a 5'09" | Olanda | Rabo Women Cycling Team [Donne] |
| 7 | Evelyn Stevens | a 5'17" | Usa | Specialized - Lululemon [Donne] |
| 8 | Ashleigh Moolman-Pasio | a 5'39" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 9 | Evgenya Vysotska | a 5'42" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 10 | Alena Amialiusik | a 7'51" | Bielorussia | Be Pink [Donne] |
| 11 | Rossella Ratto | a 8'21" | Italia | Hitec Products UCK [Donne] |
| 12 | Tiffany Cromwell | a 8'39" | Australia | Orica - AIS [Donne] |
| 13 | Anna Sanchis Chafer | a 9'25" | Spagna | Bizkaia - Durango [Donne] |
| 14 | Tatiana Antoshina | a 9'43" | Russia | MCipollini Giordana [Donne] |
| 15 | Megan Guarnier | a 9'56" | Usa | Rabo Women Cycling Team [Donne] |
| 16 | Linda Melanie Villumsen Serup | a 10'37" | Danimarca | Wiggle Honda Pro Cycling Team [Donne] |
| 17 | Georgia Williams | a 11'37" | Nuova Zelanda | Be Pink [Donne] |
| 18 | Jennifer Fiori | a 13'21" | Italia | Top Girls Fassa Bortolo [Donne] |
| 19 | Jessie Daams | a 13'54" | Belgio | Boels - Dolmans Cyclingteam [Donne] |
| 20 | Anna Van der Breggen | a 14'21" | Olanda | Sengers Ladies Cycling Team [Donne] |
| 21 | Edwige Pitel | a 14'30" | Francia | S.C. Michela Fanini Rox [Donne] |
| 22 | Ruth Corset | a 16'14" | Australia | Bizkaia - Durango [Donne] |
| 23 | Véronique Fortin | a 18'40" | Canada | Pasta Zara - Cogeas - Manhattan [Donne] |
| 24 | Amanda Spratt | a 19'23" | Australia | Orica - AIS [Donne] |
| 25 | Carlee Taylor | a 20'00" | Australia | Lotto Belisol Ladies [Donne] |
| 26 | Dalia Muccioli | a 21'18" | Italia | Be Pink [Donne] |
| 27 | Eleonora Van Dijk | a 21'25" | Olanda | Specialized - Lululemon [Donne] |
| 28 | Alexandra Burchenkova | a 21'51" | Russia | RusVelo [Donne] |
| 29 | Pauline Ferrand-Prévot | a 23'03" | Francia | Rabo Women Cycling Team [Donne] |
| 30 | Valentina Carretta | a 24'02" | Italia | MCipollini Giordana [Donne] |
