| 1 | Mara Abbott | in 13h58'43" | Usa | Exergy Twenty16 [Donne] |
| 2 | Tatiana Guderzo | a 1'27" | Italia | MCipollini Giordana [Donne] |
| 3 | Fabiana Luperini | a 1'34" | Italia | Faren - Kuota [Donne] |
| 4 | Claudia Häusler Lichtenberg | a 2'27" | Germania | TIBCO - To The Top [Donne] |
| 5 | Francesca Cauz | a 2'30" | Italia | Top Girls Fassa Bortolo [Donne] |
| 6 | Shara Gillow | a 2'54" | Australia | Orica - AIS [Donne] |
| 7 | Marianne Vos | a 3'20" | Olanda | Rabo Women Cycling Team [Donne] |
| 8 | Evelyn Stevens | a 3'35" | Usa | Specialized - Lululemon [Donne] |
| 9 | Ashleigh Moolman-Pasio | a 3'37" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 10 | Evgenya Vysotska | a 3'39" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 11 | Rossella Ratto | a 5'23" | Italia | Hitec Products UCK [Donne] |
| 12 | Alena Amialiusik | a 5'39" | Bielorussia | Be Pink [Donne] |
| 13 | Tiffany Cromwell | a 5'48" | Australia | Orica - AIS [Donne] |
| 14 | Tatiana Antoshina | a 6'20" | Russia | MCipollini Giordana [Donne] |
| 15 | Anna Sanchis Chafer | a 6'26" | Spagna | Bizkaia - Durango [Donne] |
| 16 | Linda Melanie Villumsen Serup | a 6'52" | Danimarca | Wiggle Honda Pro Cycling Team [Donne] |
| 17 | Megan Guarnier | a 7'07" | Usa | Rabo Women Cycling Team [Donne] |
| 18 | Georgia Williams | a 8'18" | Nuova Zelanda | Be Pink [Donne] |
| 19 | Jessie Daams | a 9'14" | Belgio | Boels - Dolmans Cyclingteam [Donne] |
| 20 | Anna Van der Breggen | a 9'27" | Olanda | Sengers Ladies Cycling Team [Donne] |
| 21 | Jennifer Fiori | a 9'47" | Italia | Top Girls Fassa Bortolo [Donne] |
| 22 | Edwige Pitel | a 9'53" | Francia | S.C. Michela Fanini Rox [Donne] |
| 23 | Elena Berlato | a 10'13" | Italia | Top Girls Fassa Bortolo [Donne] |
| 24 | Ruth Corset | a 11'26" | Australia | Bizkaia - Durango [Donne] |
| 25 | Alexandra Burchenkova | a 12'01" | Russia | RusVelo [Donne] |
| 26 | Amanda Spratt | a 12'20" | Australia | Orica - AIS [Donne] |
| 27 | Véronique Fortin | a 13'11" | Canada | Pasta Zara - Cogeas - Manhattan [Donne] |
| 28 | Eleonora Van Dijk | a 13'16" | Olanda | Specialized - Lululemon [Donne] |
| 29 | Ingrid Drexel | a 13'44" | Messico | Pasta Zara - Cogeas - Manhattan [Donne] |
| 30 | Carlee Taylor | a 14'24" | Australia | Lotto Belisol Ladies [Donne] |
