| 1 | Marianne Vos | in 2h58'04" | Olanda | Nederland Bloeit [Donne] |
| 2 | Emma Pooley | a 19" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 3 | Judith Arndt | a 2'50" | Germania | HTC Highroad Women [Donne] |
| 4 | Rasa Leleivyte | a 2'50" | Lituania | Vaiano Solaristech [Donne] |
| 5 | Emma Johansson | a 2'50" | Svezia | Hitec Products - UCK [Donne] |
| 6 | Ruth Corset | a 2'50" | Australia | Bizkaia - Durango [Donne] |
| 7 | Tatiana Guderzo | a 2'50" | Italia | MCipollini - Giambenini [Donne] |
| 8 | Sylwia Kapusta | a 2'59" | Polonia | Gauss [Donne] |
| 9 | Tatiana Antoshina | a 3'12" | Russia | Gauss [Donne] |
| 10 | Fabiana Luperini | a 3'24" | Italia | MCipollini - Giambenini [Donne] |
| 11 | Elena Berlato | a 3'28" | Italia | Top Girls Fassa Bortolo [Donne] |
| 12 | Evelyn Stevens | a 3'28" | Usa | HTC Highroad Women [Donne] |
| 13 | Trixi Worrack | a 3'33" | Germania | AA Drink - Leontien.nl Cycling Team [Donne] |
| 14 | Mara Abbott | a 3'35" | Usa | Diadora - Pasta Zara [Donne] |
| 15 | Elisa Longo Borghini | a 3'56" | Italia | Top Girls Fassa Bortolo [Donne] |
| 16 | Olga Zabelinskaya | a 4'11" | Russia | Diadora - Pasta Zara [Donne] |
| 17 | Sharon Laws | a 4'22" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 18 | Shara Gillow | a 4'22" | Australia | Bizkaia - Durango [Donne] |
| 19 | Grace Verbeke | a 4'25" | Belgio | Topsport Vlaanderen 2012 - Ridley Team [Donne] |
| 20 | Irene Van den Broek | a 4'26" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 21 | Christel Ferrier-Bruneau | a 4'30" | Francia | Gauss [Donne] |
| 22 | Linda Melanie Villumsen Serup | a 4'37" | Danimarca | AA Drink - Leontien.nl Cycling Team [Donne] |
| 23 | Polona Batagelj | a 4'49" | Slovenia | Bizkaia - Durango [Donne] |
| 24 | Vicki Whitelaw Eustace | a 5'06" | Australia | Lotto Honda Team [Donne] |
| 25 | Noemi Cantele | a 5'42" | Italia | Garmin - Cervélo [Donne] |
| 26 | Tetyana Riabchenko | a 5'55" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 27 | Nina Ovcharenko | a 6'13" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 28 | Andrea Graus | a 6'13" | Austria | Kleo Ladies Team [Donne] |
| 29 | Andrea Dvorak | a 6'13" | Usa | Forno d'Asolo Colavita [Donne] |
| 30 | Ashleigh Moolman-Pasio | a 6'13" | Sudafrica | Lotto Honda Team [Donne] |
