| 31 | Shelley Olds | a 1'20" | Usa | Alé - Cipollini - Galassia [Donne] |
| 32 | Lotta Lepistö | a 1'22" | Finlandia | Bigla Cycling Team [Donne] |
| 33 | Katarzyna Niewiadoma | a 1'23" | Polonia | Rabo Liv Women Cycling Team [Donne] |
| 34 | Joëlle Numainville | a 1'27" | Canada | Lotto Belisol Ladies [Donne] |
| 35 | Maria Giulia Confalonieri | a 1'29" | Italia | Estado de México - Faren [Donne] |
| 36 | Megan Guarnier | a 1'29" | Usa | Boels - Dolmans Cyclingteam [Donne] |
| 37 | Joanne Kiesanowski | a 1'30" | Nuova Zelanda | TIBCO - To The Top [Donne] |
| 38 | Simona Frapporti | a 1'32" | Italia | Astana - Be Pink [Donne] |
| 39 | Amanda Miller | a 1'35" | Usa | TIBCO - To The Top [Donne] |
| 40 | Elena Berlato | a 1'36" | Italia | Alé - Cipollini - Galassia [Donne] |
| 41 | Lauren Komanski | a 1'38" | Usa | |
| 42 | Beatrice Bartelloni | a 1'40" | Italia | Wiggle Honda [Donne] |
| 43 | Christine Majerus | a 1'41" | Lussemburgo | Boels - Dolmans Cyclingteam [Donne] |
| 44 | Marijn De Vries | a 1'42" | Olanda | Team Giant - Shimano [Donne] |
| 45 | Ksenia Dobrynina | a 1'46" | Russia | Astana - Be Pink [Donne] |
| 46 | Barbara Guarischi | a 1'46" | Italia | Alé - Cipollini - Galassia [Donne] |
| 47 | Kendall Ryan | a 1'47" | Usa | TIBCO - To The Top [Donne] |
| 48 | Kelly Druyts | a 1'48" | Belgio | Topsport Vlaanderen - Pro-Duo - Ridley [Donne] |
| 49 | Jessie Daams | a 1'50" | Belgio | Boels - Dolmans Cyclingteam [Donne] |
| 50 | Susanna Zorzi | a 1'51" | Italia | Astana - Be Pink [Donne] |
| 51 | Emilie Moberg | a 1'51" | Norvegia | Hitec Products UCK [Donne] |
| 52 | Amy Pieters | a 1'52" | Olanda | Team Giant - Shimano [Donne] |
| 53 | Nike Beckeringh | a 1'56" | Olanda | |
| 54 | Anouska Koster | a 1'58" | Olanda | Futurumshop.nl - Zannata [Donne] |
| 55 | Natalie Van Gogh | a 2'00" | Olanda | Parkhotel Valkenburg Continental Team [Donne] |
| 56 | Jasmin Glaesser | a 2'00" | Canada | TIBCO - To The Top [Donne] |
| 57 | Sara Mustonen | a 2'01" | Svezia | Team Giant - Shimano [Donne] |
| 58 | Moniek Tenniglo | a 2'03" | Olanda | Rabo Liv Women Cycling Team [Donne] |
| 59 | Mascha Pijnenborg | a 2'03" | Olanda | Futurumshop.nl - Zannata [Donne] |
| 60 | Rozanne Slik | a 2'05" | Olanda | Parkhotel Valkenburg Continental Team [Donne] |
