| 31 | Ane Santesteban Gonzalez | a 3'35" | Spagna | Alé - Cipollini - Galassia [Donne] |
| 32 | Olena Oliynyk | a 3'38" | Ucraina | Forno d'Asolo - Astute - Manhattan [Donne] |
| 33 | Eleonora Van Dijk | a 3'40" | Olanda | Boels - Dolmans Cyclingteam [Donne] |
| 34 | Tatiana Antoshina | a 3'42" | Russia | RusVelo [Donne] |
| 35 | Tatiana Guderzo | a 4'08" | Italia | Alé - Cipollini - Galassia [Donne] |
| 36 | Chiara Pierobon | a 4'35" | Italia | Top Girls Fassa Bortolo [Donne] |
| 37 | Marcia Fernandes Silva | a 4'41" | Brasile | Bizkaia - Durango [Donne] |
| 38 | Alice Maria Arzuffi | a 4'45" | Italia | Astana - Be Pink [Donne] |
| 39 | Tiffany Cromwell | a 4'46" | Australia | Specialized - Lululemon [Donne] |
| 40 | Dalia Muccioli | a 4'47" | Italia | Astana - Be Pink [Donne] |
| 41 | Lieselot Decroix | a 4'51" | Belgio | Lotto Belisol Ladies [Donne] |
| 42 | Lucinda Brand | a 4'58" | Olanda | Rabo Liv Women Cycling Team [Donne] |
| 43 | Roxane Knetemann | a 4'58" | Olanda | Rabo Liv Women Cycling Team [Donne] |
| 44 | Chantal Blaak | a 4'58" | Olanda | Specialized - Lululemon [Donne] |
| 45 | Amanda Spratt | a 4'58" | Australia | Orica - AIS [Donne] |
| 46 | Sharon Laws | a 5'42" | Gran Bretagna | Unitedhealthcare Pro Cycling Team [Donne] |
| 47 | Malgorzata Jasinska | a 5'50" | Polonia | Alé - Cipollini - Galassia [Donne] |
| 48 | Lija Laizane | a 5'54" | Lettonia | Vaiano Fondriest [Donne] |
| 49 | Serika Guluma | a 6'06" | Colombia | Vaiano Fondriest [Donne] |
| 50 | Daiva Tuslaite | a 6'09" | Lituania | Forno d'Asolo - Astute - Manhattan [Donne] |
| 51 | Jessenia Meneses | a 6'09" | Colombia | Forno d'Asolo - Astute - Manhattan [Donne] |
| 52 | Ursa Pintar | a 6'15" | Slovenia | BTC City Ljubljana [Donne] |
| 53 | Uenia Fernandes da Souza | a 6'29" | Brasile | Estado de México - Faren [Donne] |
| 54 | Azzurra D'Intino | a 6'30" | Italia | S.C. Michela Fanini Rox [Donne] |
| 55 | Peta Mullens | a 6'39" | Australia | Wiggle Honda [Donne] |
| 56 | Aizhan Zhaparova | a 6'40" | Russia | RusVelo [Donne] |
| 57 | Irene San Sebastian Lasa | a 6'50" | Spagna | Bizkaia - Durango [Donne] |
| 58 | Sarah Rijkes | a 6'54" | Austria | Lotto Belisol Ladies [Donne] |
| 59 | Carolina Rodríguez Gutiérrez | a 6'54" | Messico | Estado de México - Faren [Donne] |
| 60 | Polona Batagelj | a 7'01" | Slovenia | BTC City Ljubljana [Donne] |
