| 31 | Serika Guluma | a 6'39" | Colombia | Vaiano Fondriest [Donne] |
| 32 | Carlee Taylor | a 6'44" | Australia | Lotto Belisol Ladies [Donne] |
| 33 | Véronique Fortin | a 6'48" | Canada | Pasta Zara - Cogeas - Manhattan [Donne] |
| 34 | Valentina Carretta | a 6'48" | Italia | MCipollini Giordana [Donne] |
| 35 | Dalia Muccioli | a 6'51" | Italia | Be Pink [Donne] |
| 36 | Elena Kuchinskaya Andreeva | a 8'18" | Russia | RusVelo [Donne] |
| 37 | Sari Saarelainen | a 9'27" | Finlandia | MCipollini Giordana [Donne] |
| 38 | Andrea Dvorak | a 10'38" | Usa | Exergy Twenty16 [Donne] |
| 39 | Anouska Koster | a 13'26" | Olanda | Team Futurumshop.nl [Donne] |
| 40 | Aude Biannic | a 13'33" | Francia | S.C. Michela Fanini Rox [Donne] |
| 41 | Roxane Knetemann | a 13'36" | Olanda | Rabo Women Cycling Team [Donne] |
| 42 | Lauren Robertson Stephens | a 13'58" | Usa | TIBCO - To The Top [Donne] |
| 43 | Adrie Visser | a 14'01" | Olanda | Boels - Dolmans Cyclingteam [Donne] |
| 44 | Annemiek Van Vleuten | a 14'06" | Olanda | Rabo Women Cycling Team [Donne] |
| 45 | Valentina Scandolara | a 14'22" | Italia | MCipollini Giordana [Donne] |
| 46 | Oxana Kozonchuk | a 14'25" | Russia | RusVelo [Donne] |
| 47 | Lucinda Brand | a 14'31" | Olanda | Rabo Women Cycling Team [Donne] |
| 48 | Lorena Maria Vargas Villamil | a 14'41" | Colombia | Pasta Zara - Cogeas - Manhattan [Donne] |
| 49 | Kristin McGrath | a 14'48" | Usa | Exergy Twenty16 [Donne] |
| 50 | Joanne Hogan | a 14'50" | Australia | Bizkaia - Durango [Donne] |
| 51 | Amy Pieters | a 15'40" | Olanda | Team Argos - Shimano [Donne] |
| 52 | Inga Cilvinaite | a 15'41" | Lituania | Pasta Zara - Cogeas - Manhattan [Donne] |
| 53 | Anastasia Chulkova | a 15'48" | Russia | RusVelo [Donne] |
| 54 | Susanna Zorzi | a 16'00" | Italia | Faren - Kuota [Donne] |
| 55 | Lauren Hall | a 16'01" | Usa | Optum presented by Kelly Benefit Strategies [Donne] |
| 56 | Romy Kasper | a 16'22" | Germania | Boels - Dolmans Cyclingteam [Donne] |
| 57 | Barbara Guarischi | a 16'27" | Italia | Vaiano Fondriest [Donne] |
| 58 | Alessandra D'Ettorre | a 16'28" | Italia | Vaiano Fondriest [Donne] |
| 59 | Giada Borgato | a 16'35" | Italia | Pasta Zara - Cogeas - Manhattan [Donne] |
| 60 | Kirsten Wild | a 16'39" | Olanda | Team Argos - Shimano [Donne] |
