| 31 | Christel Ferrier-Bruneau | a 5'24" | Francia | Faren - Kuota [Donne] |
| 32 | Valentina Carretta | a 5'30" | Italia | MCipollini Giordana [Donne] |
| 33 | Dalia Muccioli | a 5'35" | Italia | Be Pink [Donne] |
| 34 | Serika Guluma | a 5'41" | Colombia | Vaiano Fondriest [Donne] |
| 35 | Carlee Taylor | a 5'50" | Australia | Lotto Belisol Ladies [Donne] |
| 36 | Elena Kuchinskaya Andreeva | a 5'59" | Russia | RusVelo [Donne] |
| 37 | Sari Saarelainen | a 8'05" | Finlandia | MCipollini Giordana [Donne] |
| 38 | Andrea Dvorak | a 8'19" | Usa | Exergy Twenty16 [Donne] |
| 39 | Lauren Robertson Stephens | a 12'08" | Usa | TIBCO - To The Top [Donne] |
| 40 | Anouska Koster | a 12'08" | Olanda | Team Futurumshop.nl [Donne] |
| 41 | Aude Biannic | a 12'15" | Francia | S.C. Michela Fanini Rox [Donne] |
| 42 | Roxane Knetemann | a 12'18" | Olanda | Rabo Women Cycling Team [Donne] |
| 43 | Kristin McGrath | a 12'22" | Usa | Exergy Twenty16 [Donne] |
| 44 | Miriam Bjørnsrud | a 13'00" | Norvegia | Hitec Products UCK [Donne] |
| 45 | Adrie Visser | a 13'01" | Olanda | Boels - Dolmans Cyclingteam [Donne] |
| 46 | Annemiek Van Vleuten | a 13'02" | Olanda | Rabo Women Cycling Team [Donne] |
| 47 | Lorena Maria Vargas Villamil | a 13'03" | Colombia | Pasta Zara - Cogeas - Manhattan [Donne] |
| 48 | Oxana Kozonchuk | a 13'11" | Russia | RusVelo [Donne] |
| 49 | Joanne Hogan | a 13'14" | Australia | Bizkaia - Durango [Donne] |
| 50 | Valentina Scandolara | a 13'17" | Italia | MCipollini Giordana [Donne] |
| 51 | Lucinda Brand | a 13'21" | Olanda | Rabo Women Cycling Team [Donne] |
| 52 | Ruth Winder | a 13'21" | Usa | |
| 53 | Barbara Guarischi | a 13'28" | Italia | Vaiano Fondriest [Donne] |
| 54 | Lauren Hall | a 13'35" | Usa | Optum presented by Kelly Benefit Strategies [Donne] |
| 55 | Trixi Worrack | a 13'35" | Germania | Specialized - Lululemon [Donne] |
| 56 | Brianna Walle | a 14'17" | Usa | Optum presented by Kelly Benefit Strategies [Donne] |
| 57 | Inga Cilvinaite | a 14'25" | Lituania | Pasta Zara - Cogeas - Manhattan [Donne] |
| 58 | Anastasia Chulkova | a 14'25" | Russia | RusVelo [Donne] |
| 59 | Alessandra D'Ettorre | a 14'32" | Italia | Vaiano Fondriest [Donne] |
| 60 | Romy Kasper | a 14'32" | Germania | Boels - Dolmans Cyclingteam [Donne] |
