| 31 | Shara Gillow | a 1'22" | Australia | Orica - AIS [Donne] |
| 32 | Christine Majerus | a 1'29" | Lussemburgo | Sengers Ladies Cycling Team [Donne] |
| 33 | Oriane Chaumet | a 1'29" | Francia | Vienne Futuroscope [Donne] |
| 34 | Aude Biannic | a 1'43" | Francia | S.C. Michela Fanini Rox [Donne] |
| 35 | Amanda Spratt | a 1'59" | Australia | Orica - AIS [Donne] |
| 36 | Lauren Hall | a 2'13" | Usa | Optum presented by Kelly Benefit Strategies [Donne] |
| 37 | Tatiana Guderzo | a 2'21" | Italia | MCipollini Giordana [Donne] |
| 38 | Tatiana Antoshina | a 2'23" | Russia | MCipollini Giordana [Donne] |
| 39 | Valentina Scandolara | a 2'24" | Italia | MCipollini Giordana [Donne] |
| 40 | Audrey Cordon | a 2'34" | Francia | Vienne Futuroscope [Donne] |
| 41 | Oxana Kozonchuk | a 2'36" | Russia | RusVelo [Donne] |
| 42 | Elena Bocharnikova | a 2'43" | Russia | RusVelo [Donne] |
| 43 | Christel Ferrier-Bruneau | a 2'51" | Francia | Faren - Kuota [Donne] |
| 44 | Sandrine Bideau | a 3'28" | Francia | Vienne Futuroscope [Donne] |
| 45 | Adrie Visser | a 4'29" | Olanda | Boels - Dolmans Cyclingteam [Donne] |
| 46 | Joanna Rowsell | a 4'29" | Gran Bretagna | Wiggle Honda Pro Cycling Team [Donne] |
| 47 | Loes Gunnewijk | a 4'32" | Olanda | Orica - AIS [Donne] |
| 48 | Annelies Van Doorslaer | a 4'32" | Belgio | Cyclelive Plus - Zannata [Donne] |
| 49 | Rachel Neylan | a 4'38" | Australia | Hitec Products UCK [Donne] |
| 50 | Lucinda Brand | a 4'46" | Olanda | Rabo Women Cycling Team [Donne] |
| 51 | Sanne Van Paassen | a 4'59" | Olanda | Rabo Women Cycling Team [Donne] |
| 52 | Carmen McNellis Small | a 4'59" | Usa | Specialized - Lululemon [Donne] |
| 53 | Anna-Bianca Schnitzmeier | a 5'01" | Germania | Wiggle Honda Pro Cycling Team [Donne] |
| 54 | Valentina Carretta | a 5'06" | Italia | MCipollini Giordana [Donne] |
| 55 | Ann Sophie Duyck | a 5'06" | Belgio | Lotto Belisol Ladies [Donne] |
| 56 | Inge Roggeman | a 5'06" | Belgio | Sengers Ladies Cycling Team [Donne] |
| 57 | Manon Souyris | a 8'16" | Francia | Vienne Futuroscope [Donne] |
| 58 | Emmanuelle Merlot | a 8'16" | Francia | Vienne Futuroscope [Donne] |
| 59 | Susanna Zorzi | a 8'16" | Italia | Faren - Kuota [Donne] |
| 60 | Alexandra Burchenkova | a 8'35" | Russia | RusVelo [Donne] |
