| 31 | Shelley Olds | a 10'55" | Usa | AA Drink - Leontien.nl Cycling Team [Donne] |
| 32 | Tatiana Antoshina | a 13'06" | Russia | Rabobank Women Team [Donne] |
| 33 | Verónica Leal Balderas | a 13'19" | Messico | S.C. Michela Fanini Rox [Donne] |
| 34 | Lucinda Brand | a 13'33" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 35 | Hanka Kupfernagel | a 14'33" | Germania | RusVelo [Donne] |
| 36 | Anna Van der Breggen | a 14'36" | Olanda | Sengers Ladies Cycling Team [Donne] |
| 37 | Inga Cilvinaite | a 14'53" | Lituania | Diadora - Pasta Zara [Donne] |
| 38 | Christel Ferrier-Bruneau | a 15'00" | Francia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 39 | Marta Tagliaferro | a 15'01" | Italia | MCipollini Giambenini [Donne] |
| 40 | Romy Kasper | a 15'08" | Germania | RusVelo [Donne] |
| 41 | Martina Ruzickova | a 15'10" | Repubblica Ceca | S.C. Michela Fanini Rox [Donne] |
| 42 | Alessandra Giuseppina Grassi Herrera | a 15'18" | Messico | Faren Honda Team [Donne] |
| 43 | Robyn De Groot | a 15'20" | Sudafrica | Lotto Belisol Ladies [Donne] |
| 44 | Oxana Kozonchuk | a 15'24" | Russia | Be Pink [Donne] |
| 45 | Sylwia Kapusta | a 15'55" | Polonia | Hitec Products - Mistral Home Cycling Team [Donne] |
| 46 | Tetyana Riabchenko | a 20'10" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 47 | Charlotte Becker | a 20'44" | Germania | Team Specialized - Lululemon [Donne] |
| 48 | Jennifer Hohl | a 20'55" | Svizzera | Faren Honda Team [Donne] |
| 49 | Ina-Yoko Teutenberg | a 21'00" | Germania | Team Specialized - Lululemon [Donne] |
| 50 | Chloe Hosking | a 21'07" | Australia | Team Specialized - Lululemon [Donne] |
| 51 | Monia Baccaille | a 21'25" | Italia | MCipollini Giambenini [Donne] |
| 52 | Susanna Zorzi | a 21'30" | Italia | MCipollini Giambenini [Donne] |
| 53 | Petra Zrimsek | a 22'03" | Slovenia | Be Pink [Donne] |
| 54 | Malgorzata Jasinska | a 23'02" | Polonia | MCipollini Giambenini [Donne] |
| 55 | Lorena Foresi | a 23'02" | Italia | S.C. Michela Fanini Rox [Donne] |
| 56 | Doris Schweizer | a 24'33" | Svizzera | Top Girls Fassa Bortolo - Servetto [Donne] |
| 57 | Elizabeth Armitstead | a 27'58" | Gran Bretagna | AA Drink - Leontien.nl Cycling Team [Donne] |
| 58 | Irina Molicheva | a 28'00" | Russia | RusVelo [Donne] |
| 59 | Silvia Valsecchi | a 28'01" | Italia | Be Pink [Donne] |
| 60 | Lisa Brennauer | a 28'05" | Germania | Team Specialized - Lululemon [Donne] |
