| 31 | Pierre Rolland | a 1'50" | Francia | Team Europcar |
| 32 | Carlos José Ochoa | a 1'50" | Venezuela | Androni Giocattoli - Venezuela |
| 33 | Cyril Gautier | a 1'50" | Francia | Team Europcar |
| 34 | Guillaume Bonnafond | a 1'50" | Francia | AG2R La Mondiale |
| 35 | Paolo Tiralongo | a 1'50" | Italia | Astana Pro Team |
| 36 | David Boily | a 2'34" | Canada | SpiderTech powered by C10 |
| 37 | Cristian Cominelli | a 2'39" | Italia | Team Idea |
| 38 | Angelo Pagani | a 2'55" | Italia | Colnago - CSF Inox |
| 39 | Matteo Rabottini | a 2'55" | Italia | Farnese Vini - Selle Italia |
| 40 | Chris Butler | a 3'11" | Usa | Champion System Pro Cycling Team |
| 41 | Marco Frapporti | a 3'35" | Italia | Team Idea |
| 42 | Kevin Seeldraeyers | a 3'35" | Belgio | Astana Pro Team |
| 43 | Tanel Kangert | a 3'43" | Estonia | Astana Pro Team |
| 44 | Janez Brajkovic | a 3'43" | Slovenia | Astana Pro Team |
| 45 | Andrey Kashechkin | a 3'43" | Kazakistan | Astana Pro Team |
| 46 | Jackson Rodríguez | a 3'43" | Venezuela | Androni Giocattoli - Venezuela |
| 47 | Brian Bach Vandborg | a 3'50" | Danimarca | SpiderTech powered by C10 |
| 48 | Vladimir Miholjevic | a 3'50" | Croazia | Acqua & Sapone |
| 49 | Reto Hollenstein | a 3'50" | Svizzera | Team NetApp |
| 50 | Stiven Fanelli | a 3'50" | Italia | Utensilnord Named |
| 51 | Danilo Di Luca | a 4'13" | Italia | Acqua & Sapone |
| 52 | Luca Ascani | a 4'19" | Italia | Farnese Vini - Selle Italia |
| 53 | Raymond Künzli | a 5'02" | Svizzera | SpiderTech powered by C10 |
| 54 | Cameron Wurf | a 5'02" | Australia | Champion System Pro Cycling Team |
| 55 | Fabio Felline | a 5'02" | Italia | Androni Giocattoli - Venezuela |
| 56 | Daniel Schorn | a 5'15" | Austria | Team NetApp |
| 57 | Simone Boifava | a 5'15" | Italia | Team Idea |
| 58 | Morris Possoni | a 5'15" | Italia | Lampre - ISD |
| 59 | Javier Alberto González Barrera | a 5'15" | Colombia | Colombia - Coldeportes |
| 60 | Pengda Jiao | a 5'15" | Cina | Champion System Pro Cycling Team |
