| 31 | Elena Berlato | a 10'09" | Italia | Top Girls Fassa Bortolo [Donne] |
| 32 | Svetlana Bubnenkova | a 10'11" | Russia | Team Project Cycling Women [Donne] |
| 33 | Verónica Leal Balderas | a 10'16" | Messico | S.C. Michela Fanini Rox [Donne] |
| 34 | Malgorzata Jasinska | a 10'32" | Polonia | S.C. Michela Fanini Rox [Donne] |
| 35 | Fabiana Luperini | a 10'41" | Italia | MCipollini - Giambenini [Donne] |
| 36 | Elizabeth Armitstead | a 11'16" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 37 | Nina Ovcharenko | a 11'18" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 38 | Kristin McGrath | a 11'28" | Usa | |
| 39 | Ally Stacher | a 11'30" | Usa | HTC Highroad Women [Donne] |
| 40 | Yulia Blindiuk | a 11'35" | Russia | MCipollini - Giambenini [Donne] |
| 41 | Mascha Pijnenborg | a 12'08" | Olanda | Dolmans Landscaping Team [Donne] |
| 42 | Stephanie Pohl | a 15'03" | Germania | |
| 43 | Anna-Bianca Schnitzmeier | a 15'20" | Germania | Abus - Nutrixxion [Donne] |
| 44 | Charlotte Becker | a 15'31" | Germania | HTC Highroad Women [Donne] |
| 45 | Trixi Worrack | a 16'15" | Germania | AA Drink - Leontien.nl Cycling Team [Donne] |
| 46 | Marta Tagliaferro | a 16'48" | Italia | MCipollini - Giambenini [Donne] |
| 47 | Lucy Martin | a 16'51" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 48 | Simona Frapporti | a 16'51" | Italia | Top Girls Fassa Bortolo [Donne] |
| 49 | Monia Baccaille | a 16'55" | Italia | MCipollini - Giambenini [Donne] |
| 50 | Tiffany Cromwell | a 16'59" | Australia | Lotto Honda Team [Donne] |
| 51 | Marta Bastianelli | a 16'59" | Italia | MCipollini - Giambenini [Donne] |
| 52 | Carmen McNellis Small | a 17'00" | Usa | TIBCO - To The Top [Donne] |
| 53 | Alessandra Borchi | a 17'00" | Italia | Gauss [Donne] |
| 54 | Lorena Foresi | a 17'06" | Italia | Gauss [Donne] |
| 55 | Elke Gebhardt | a 17'06" | Germania | |
| 56 | Giorgia Bronzini | a 17'07" | Italia | Forno d'Asolo Colavita [Donne] |
| 57 | Alessandra D'Ettorre | a 17'08" | Italia | Forno d'Asolo Colavita [Donne] |
| 58 | Romy Kasper | a 17'10" | Germania | Kuota Speed Kueens [Donne] |
| 59 | Eleonora Patuzzo | a 17'11" | Italia | Diadora - Pasta Zara [Donne] |
| 60 | Ivanna Borovichenko | a 17'13" | Ucraina | |
