| 31 | Tetyana Riabchenko | a 10'18" | Ucraina | Forno d'Asolo Colavita [Donne] |
| 32 | Elena Berlato | a 10'28" | Italia | Top Girls Fassa Bortolo [Donne] |
| 33 | Svetlana Bubnenkova | a 10'30" | Russia | Team Project Cycling Women [Donne] |
| 34 | Verónica Leal Balderas | a 10'35" | Messico | S.C. Michela Fanini Rox [Donne] |
| 35 | Malgorzata Jasinska | a 10'58" | Polonia | S.C. Michela Fanini Rox [Donne] |
| 36 | Fabiana Luperini | a 11'00" | Italia | MCipollini - Giambenini [Donne] |
| 37 | Nina Ovcharenko | a 11'37" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 38 | Elizabeth Armitstead | a 11'42" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 39 | Kristin McGrath | a 11'47" | Usa | |
| 40 | Ally Stacher | a 11'49" | Usa | HTC Highroad Women [Donne] |
| 41 | Yulia Blindiuk | a 11'54" | Russia | MCipollini - Giambenini [Donne] |
| 42 | Tjasa Rutar | a 12'33" | Slovenia | |
| 43 | Mascha Pijnenborg | a 12'34" | Olanda | Dolmans Landscaping Team [Donne] |
| 44 | Stephanie Pohl | a 15'22" | Germania | |
| 45 | Anna-Bianca Schnitzmeier | a 15'39" | Germania | Abus - Nutrixxion [Donne] |
| 46 | Charlotte Becker | a 15'50" | Germania | HTC Highroad Women [Donne] |
| 47 | Joanne Hogan | a 16'03" | Australia | |
| 48 | Trixi Worrack | a 16'34" | Germania | AA Drink - Leontien.nl Cycling Team [Donne] |
| 49 | Katie Colclough | a 17'01" | Gran Bretagna | HTC Highroad Women [Donne] |
| 50 | Marta Tagliaferro | a 17'14" | Italia | MCipollini - Giambenini [Donne] |
| 51 | Lucy Martin | a 17'17" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 52 | Simona Frapporti | a 17'17" | Italia | Top Girls Fassa Bortolo [Donne] |
| 53 | Tiffany Cromwell | a 17'18" | Australia | Lotto Honda Team [Donne] |
| 54 | Carmen McNellis Small | a 17'19" | Usa | TIBCO - To The Top [Donne] |
| 55 | Lorena Foresi | a 17'25" | Italia | Gauss [Donne] |
| 56 | Elke Gebhardt | a 17'25" | Germania | |
| 57 | Monia Baccaille | a 17'25" | Italia | MCipollini - Giambenini [Donne] |
| 58 | Marta Bastianelli | a 17'25" | Italia | MCipollini - Giambenini [Donne] |
| 59 | Alessandra Borchi | a 17'26" | Italia | Gauss [Donne] |
| 60 | Robyn De Groot | a 17'29" | Sudafrica | Lotto Honda Team [Donne] |
