| 31 | Anders Lund [Dan] | a 1'18" | Danimarca | Leopard Trek |
| 32 | Bruno Manuel Silva Pires | a 1'19" | Portogallo | Leopard Trek |
| 33 | Jens Voigt | a 1'20" | Germania | Leopard Trek |
| 34 | Rubens Bertogliati | a 1'20" | Svizzera | Team Type 1 - Sanofi Aventis |
| 35 | Lucas Euser | a 1'23" | Usa | Team SpiderTech powered by C10 |
| 36 | George Bennett | a 1'24" | Nuova Zelanda | Trek Livestrong U23 |
| 37 | Jaime Alberto Castañeda Ortega | a 1'24" | Colombia | EPM - UNE |
| 38 | Danny Pate | a 1'26" | Usa | HTC - Highroad |
| 39 | Alex Hagman | a 1'26" | Usa | Jelly Belly Cycling |
| 40 | Daniel Oss | a 1'28" | Italia | Liquigas - Cannondale |
| 41 | Flavio De Luna Davila | a 1'29" | Canada | Team SpiderTech powered by C10 |
| 42 | Andrès Miguel Diaz Corrales | a 1'31" | Colombia | Team Exergy |
| 43 | Rafael Anibal Montiel Cuellar | a 1'32" | Colombia | Gobiernación de Antioquia - Indeportes Antioquia |
| 44 | Andy Schleck | a 1'38" | Lussemburgo | Leopard Trek |
| 45 | Eduard Alexander Beltrán Suárez | a 1'44" | Colombia | EPM - UNE |
| 46 | Alexander Efimkin | a 1'52" | Russia | Team Type 1 - Sanofi Aventis |
| 47 | Cristhian Montoya Giraldo | a 1'54" | Colombia | Gobiernación de Antioquia - Indeportes Antioquia |
| 48 | Sylwester Szmyd | a 2'04" | Polonia | Liquigas - Cannondale |
| 49 | Christopher Jones | a 2'09" | Usa | Unitedhealthcare Pro Cycling |
| 50 | Hayden Roulston | a 2'09" | Nuova Zelanda | HTC - Highroad |
| 51 | Edward King | a 2'12" | Usa | Liquigas - Cannondale |
| 52 | Walter Fernando Pedraza Morales | a 2'17" | Colombia | EPM - UNE |
| 53 | André Steensen | a 2'19" | Danimarca | Saxo Bank SunGard |
| 54 | Javier Mejías Leal | a 2'20" | Spagna | Team Type 1 - Sanofi Aventis |
| 55 | Juraj Sagan | a 2'20" | Slovacchia | Liquigas - Cannondale |
| 56 | Valeriy Kobzarenko | a 2'23" | Ucraina | Team Type 1 - Sanofi Aventis |
| 57 | Ben Hermans | a 2'25" | Belgio | Team RadioShack |
| 58 | Edward Stiver Ortiz Caro | a 2'27" | Colombia | EPM - UNE |
| 59 | Carlos Fernando Piamonte Rodríguez | a 2'28" | Colombia | EPM - UNE |
| 60 | Matt Cooke | a 2'30" | Usa | Team Exergy |
