| 31 | Elia Viviani | a 5'26" | Italia | Liquigas - Cannondale |
| 32 | Óscar Mauricio Álvarez Paniagua | a 5'43" | Colombia | Gobiernación de Antioquia - Indeportes Antioquia |
| 33 | Giampaolo Cheula | a 5'44" | Italia | Geox - TMC |
| 34 | Brent Bookwalter | a 5'54" | Usa | BMC Racing Team |
| 35 | Cristhian Montoya Giraldo | a 5'56" | Colombia | Gobiernación de Antioquia - Indeportes Antioquia |
| 36 | Danny Pate | a 6'09" | Usa | HTC - Highroad |
| 37 | David Gutiérrez Gutiérrez | a 6'12" | Spagna | Geox - TMC |
| 38 | Peter Stetina | a 6'46" | Usa | Team Garmin - Cervélo |
| 39 | Rob Britton | a 6'58" | Canada | Bissell Cycling |
| 40 | Christopher Jones | a 7'04" | Usa | Unitedhealthcare Pro Cycling |
| 41 | Alexander Efimkin | a 7'07" | Russia | Team Type 1 - Sanofi Aventis |
| 42 | Robert Partridge | a 7'33" | Gran Bretagna | Endura Racing |
| 43 | Alejandro Ramírez Calderon | a 8'17" | Colombia | Gobiernación de Antioquia - Indeportes Antioquia |
| 44 | Paul Voss | a 8'17" | Germania | Endura Racing |
| 45 | Davide Frattini | a 8'30" | Italia | Unitedhealthcare Pro Cycling |
| 46 | Daniel Summerhill | a 8'34" | Usa | Chipotle Development Team |
| 47 | David Zabriskie | a 8'39" | Usa | Team Garmin - Cervélo |
| 48 | Philip Deignan | a 8'40" | Irlanda | Team RadioShack |
| 49 | Paolo Longo Borghini | a 8'51" | Italia | Liquigas - Cannondale |
| 50 | Tom Zirbel | a 9'43" | Usa | Jamis - Sutter Home |
| 51 | Alexander Wetterhall | a 9'47" | Svezia | Endura Racing |
| 52 | Maurizio Gorato | a 9'56" | Italia | Brunero - Camel - Pedalando in Langa [Dilettanti] |
| 53 | Jason Donald | a 10'41" | Usa | Kelly Benefit Strategies - Optumhealth |
| 54 | Edward King | a 10'41" | Usa | Liquigas - Cannondale |
| 55 | Chris Butler | a 10'57" | Usa | BMC Racing Team |
| 56 | George Bennett | a 10'57" | Nuova Zelanda | Trek Livestrong U23 |
| 57 | James Driscoll | a 11'14" | Usa | Jamis - Sutter Home |
| 58 | Rubens Bertogliati | a 11'14" | Svizzera | Team Type 1 - Sanofi Aventis |
| 59 | Lachlan Norris | a 11'20" | Australia | Drapac Cycling |
| 60 | William Dugan | a 11'25" | Usa | Team Type 1 - Sanofi Aventis |
