| 31 | Elizabeth Armitstead | a 5'44" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 32 | Julia Martisova | a 5'49" | Russia | Gauss [Donne] |
| 33 | Sara Mustonen | a 5'58" | Svezia | Hitec Products - UCK [Donne] |
| 34 | Elena Kuchinskaya Andreeva | a 6'26" | Russia | Gauss [Donne] |
| 35 | Madelene Olsson | a 6'33" | Svezia | Alriksson Go:Green [Donne] |
| 36 | Ina-Yoko Teutenberg | a 6'44" | Germania | HTC Highroad Women [Donne] |
| 37 | Noemi Cantele | a 6'48" | Italia | Garmin - Cervélo [Donne] |
| 38 | Isabelle Söderberg | a 7'08" | Svezia | Alriksson Go:Green [Donne] |
| 39 | Malin Rydlund | a 7'42" | Svezia | Alriksson Go:Green [Donne] |
| 40 | Lisa Brennauer | a 7'43" | Germania | Hitec Products - UCK [Donne] |
| 41 | Marijn De Vries | a 7'48" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 42 | Lucinda Brand | a 7'51" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 43 | Katie Colclough | a 8'05" | Gran Bretagna | HTC Highroad Women [Donne] |
| 44 | Oksana Kashchishina | a 8'18" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 45 | Amy Pieters | a 8'38" | Olanda | |
| 46 | Nina Ovcharenko | a 8'40" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 47 | Cristina Alcalde Huertanos | a 9'02" | Spagna | Bizkaia - Durango [Donne] |
| 48 | Claudia Häusler Lichtenberg | a 9'03" | Germania | Diadora - Pasta Zara [Donne] |
| 49 | Marissa Van der Merwe Stander | a 9'37" | Sudafrica | Lotto Honda Team [Donne] |
| 50 | Romy Kasper | a 9'48" | Germania | Kuota Speed Kueens [Donne] |
| 51 | Anna Sanchis Chafer | a 11'35" | Spagna | Bizkaia - Durango [Donne] |
| 52 | Carla Ryan | a 11'45" | Australia | Garmin - Cervélo [Donne] |
| 53 | Valentina Carretta | a 11'47" | Italia | Top Girls Fassa Bortolo [Donne] |
| 54 | Charlotte Becker | a 11'58" | Germania | HTC Highroad Women [Donne] |
| 55 | Chantal Blaak | a 14'01" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 56 | Martina Ruzickova | a 14'11" | Repubblica Ceca | S.C. Michela Fanini Rox [Donne] |
| 57 | Jennie Stenerhag | a 14'32" | Svezia | Alriksson Go:Green [Donne] |
| 58 | Joanne Hogan | a 18'24" | Australia | |
| 59 | Stephanie Pohl | a 18'53" | Germania | |
| 60 | Eleonora Patuzzo | a 18'59" | Italia | Diadora - Pasta Zara [Donne] |
