| 31 | Anna-Bianca Schnitzmeier | a 1'17" | Germania | Abus - Nutrixxion [Donne] |
| 32 | Romy Kasper | a 1'17" | Germania | Kuota Speed Kueens [Donne] |
| 33 | Alexandra Burchenkova | a 1'24" | Russia | |
| 34 | Sylwia Kapusta | a 1'26" | Polonia | Gauss [Donne] |
| 35 | Roxane Knetemann | a 1'27" | Olanda | |
| 36 | Madelene Olsson | a 1'29" | Svezia | Alriksson Go:Green [Donne] |
| 37 | Carla Ryan | a 1'30" | Australia | Garmin - Cervélo [Donne] |
| 38 | Nina Ovcharenko | a 1'39" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 39 | Shara Gillow | a 1'42" | Australia | Bizkaia - Durango [Donne] |
| 40 | Sofie De Vuyst | a 1'46" | Belgio | Lotto Honda Team [Donne] |
| 41 | Amanda Spratt | a 1'58" | Australia | |
| 42 | Ashleigh Moolman-Pasio | a 2'26" | Sudafrica | Lotto Honda Team [Donne] |
| 43 | Irina Molicheva | a 2'49" | Russia | Team Project Cycling Women [Donne] |
| 44 | Petra Dijkman | a 3'18" | Olanda | Dolmans Landscaping Team [Donne] |
| 45 | Jessie Daams | a 3'38" | Belgio | Garmin - Cervélo [Donne] |
| 46 | Lise Nöstvold | a 3'43" | Norvegia | Hitec Products - UCK [Donne] |
| 47 | Elizabeth Armitstead | a 3'43" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 48 | Chantal Blaak | a 3'48" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 49 | Loes Gunnewijk | a 3'55" | Olanda | Nederland Bloeit [Donne] |
| 50 | Trixi Worrack | a 3'55" | Germania | AA Drink - Leontien.nl Cycling Team [Donne] |
| 51 | Evelyn Stevens | a 3'55" | Usa | HTC Highroad Women [Donne] |
| 52 | Sandrine Bideau | a 4'10" | Francia | |
| 53 | Noemi Cantele | a 4'15" | Italia | Garmin - Cervélo [Donne] |
| 54 | Sara Mustonen | a 4'20" | Svezia | Hitec Products - UCK [Donne] |
| 55 | Theresa Cliff-Ryan | a 5'07" | Usa | Forno d'Asolo Colavita [Donne] |
| 56 | Marieke Van Wanroij | a 5'07" | Olanda | Nederland Bloeit [Donne] |
| 57 | Tiffany Cromwell | a 5'07" | Australia | Lotto Honda Team [Donne] |
| 58 | Sarah-Lena Hofmann | a 5'11" | Germania | |
| 59 | Samantha Galassi | a 5'18" | Italia | MCipollini - Giambenini [Donne] |
| 60 | Serena Sheridan | a 5'19" | Nuova Zelanda | TIBCO - To The Top [Donne] |
