| 31 | Polona Batagelj | a 3'13" | Slovenia | Bizkaia - Durango [Donne] |
| 32 | Catherine Hare Williamson | a 3'41" | Gran Bretagna | |
| 33 | Giorgia Bronzini | a 4'06" | Italia | Gauss RDZ Ormu [Donne] |
| 34 | Luise Keller | a 4'17" | Germania | HTC - Columbia Women [Donne] |
| 35 | Julia Martisova | a 4'18" | Russia | Gauss RDZ Ormu [Donne] |
| 36 | Rasa Leleivyte | a 4'23" | Lituania | Safi - Pasta Zara [Donne] |
| 37 | Loes Gunnewijk | a 4'26" | Olanda | Nederland Bloeit [Donne] |
| 38 | Trixi Worrack | a 4'31" | Germania | Noris Cycling [Donne] |
| 39 | Edwige Pitel | a 4'36" | Francia | S.C. Michela Fanini Record Rox [Donne] |
| 40 | Martina Ruzickova | a 4'41" | Repubblica Ceca | S.C. Michela Fanini Record Rox [Donne] |
| 41 | Uenia Fernandes da Souza | a 4'44" | Brasile | USC Chirio Forno d'Asolo [Donne] |
| 42 | Malgorzata Jasinska | a 4'44" | Polonia | Team System Data [Donne] |
| 43 | Noortje Tabak | a 4'57" | Olanda | Nederland Bloeit [Donne] |
| 44 | Belen Lopez Morales | a 4'58" | Spagna | Lointek [Donne] |
| 45 | Oksana Kashchishina | a 5'19" | Ucraina | Team System Data [Donne] |
| 46 | Chiara Vanni | a 5'20" | Italia | Vaiano Solaristech [Donne] |
| 47 | Regina Bruins | a 6'07" | Olanda | Cervélo Test Team [Donne] |
| 48 | Roxane Knetemann | a 6'21" | Olanda | |
| 49 | Sara Mustonen | a 6'41" | Svezia | Hitec Products UCK [Donne] |
| 50 | Iris Slappendel | a 6'51" | Olanda | Cervélo Test Team [Donne] |
| 51 | Sigrid Corneo | a 7'08" | Italia | Top Girls Fassa Bortolo - Ghezzi [Donne] |
| 52 | Marie Lindberg | a 7'09" | Svezia | Red Sun Cycling Team [Donne] |
| 53 | Madeleine Sandig | a 7'16" | Germania | Noris Cycling [Donne] |
| 54 | Ina-Yoko Teutenberg | a 7'20" | Germania | HTC - Columbia Women [Donne] |
| 55 | Luisa Tamanini | a 7'24" | Italia | USC Chirio Forno d'Asolo [Donne] |
| 56 | Lucy Martin | a 7'31" | Gran Bretagna | |
| 57 | Chantal Blaak | a 7'48" | Olanda | Leontien.nl [Donne] |
| 58 | Madelene Olsson | a 8'32" | Svezia | Alriksson Go:Green [Donne] |
| 59 | Romy Kasper | a 8'41" | Germania | Noris Cycling [Donne] |
| 60 | Hanna Solovey | a 8'44" | Ucraina | |
