| 1 | Judith Arndt | in 4h16'33" | Germania | HTC Highroad Women [Donne] |
| 2 | Tatiana Guderzo | a 10" | Italia | MCipollini - Giambenini [Donne] |
| 3 | Emma Pooley | a 12" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 4 | Rasa Leleivyte | a 20" | Lituania | Vaiano Solaristech [Donne] |
| 5 | Emma Johansson | a 22" | Svezia | Hitec Products - UCK [Donne] |
| 6 | Rossella Callovi | a 1'02" | Italia | MCipollini - Giambenini [Donne] |
| 7 | Ina-Yoko Teutenberg | a 1'15" | Germania | HTC Highroad Women [Donne] |
| 8 | Polona Batagelj | a 1'29" | Slovenia | Bizkaia - Durango [Donne] |
| 9 | Eva Lechner | a 1'32" | Italia | Centro Sportivo Esercito [Donne] |
| 10 | Noemi Cantele | a 1'32" | Italia | Garmin - Cervélo [Donne] |
| 11 | Sharon Laws | a 1'34" | Gran Bretagna | Garmin - Cervélo [Donne] |
| 12 | Olga Zabelinskaya | a 1'34" | Russia | Diadora - Pasta Zara [Donne] |
| 13 | Elisa Longo Borghini | a 1'38" | Italia | Top Girls Fassa Bortolo [Donne] |
| 14 | Sylwia Kapusta | a 1'41" | Polonia | Gauss [Donne] |
| 15 | Shara Gillow | a 1'41" | Australia | Bizkaia - Durango [Donne] |
| 16 | Natalia Boyarskaya | a 1'48" | Russia | Team Project Cycling Women [Donne] |
| 17 | Fabiana Luperini | a 2'37" | Italia | MCipollini - Giambenini [Donne] |
| 18 | Elena Berlato | a 2'50" | Italia | Top Girls Fassa Bortolo [Donne] |
| 19 | Martine Bras | a 3'55" | Olanda | Dolmans Landscaping Team [Donne] |
| 20 | Mascha Pijnenborg | a 4'00" | Olanda | Dolmans Landscaping Team [Donne] |
| 21 | Svetlana Bubnenkova | a 4'00" | Russia | Team Project Cycling Women [Donne] |
| 22 | Andrea Graus | a 4'00" | Austria | Kleo Ladies Team [Donne] |
| 23 | Adrie Visser | a 4'01" | Olanda | HTC Highroad Women [Donne] |
| 24 | Nina Ovcharenko | a 4'02" | Ucraina | S.C. Michela Fanini Rox [Donne] |
| 25 | Ludivine Henrion | a 4'02" | Belgio | Lotto Honda Team [Donne] |
| 26 | Julia Martisova | a 4'04" | Russia | Gauss [Donne] |
| 27 | Lisa Brennauer | a 4'04" | Germania | Hitec Products - UCK [Donne] |
| 28 | Vicki Whitelaw Eustace | a 4'04" | Australia | Lotto Honda Team [Donne] |
| 29 | Anne De Wildt | a 4'04" | Olanda | AA Drink - Leontien.nl Cycling Team [Donne] |
| 30 | Malgorzata Jasinska | a 4'07" | Polonia | S.C. Michela Fanini Rox [Donne] |
