| 1 | Marianne Vos | in 3h27'37" | Olanda | Nederland Bloeit [Donne] |
| 2 | Martine Bras | st | Olanda | Gauss RDZ Ormu [Donne] |
| 3 | Emma Johansson | st | Svezia | Red Sun Cycling Team [Donne] |
| 4 | Ruth Corset | st | Australia | Team TIBCO [Donne] |
| 5 | Noemi Cantele | st | Italia | HTC - Columbia Women [Donne] |
| 6 | Nicole Cooke | st | Gran Bretagna | |
| 7 | Elena Berlato | st | Italia | Top Girls Fassa Bortolo - Ghezzi [Donne] |
| 8 | Judith Arndt | st | Germania | HTC - Columbia Women [Donne] |
| 9 | Tatiana Guderzo | st | Italia | Team Valdarno [Donne] |
| 10 | Olga Zabelinskaya | a 5" | Russia | Safi - Pasta Zara [Donne] |
| 11 | Andrea Bosman | a 14" | Olanda | Leontien.nl [Donne] |
| 12 | Grace Verbeke | a 32" | Belgio | Lotto Ladies Team [Donne] |
| 13 | Rachel Neylan | a 1'06" | Australia | Team System Data [Donne] |
| 14 | Emma Pooley | a 1'06" | Gran Bretagna | Cervélo Test Team [Donne] |
| 15 | Irina Molicheva | a 2'04" | Russia | Fenixs - Petrogradets [Donne] |
| 16 | Amanda Spratt | a 4'25" | Australia | |
| 17 | Ludivine Henrion | a 4'25" | Belgio | Red Sun Cycling Team [Donne] |
| 18 | Sinead Miller | a 4'25" | Usa | |
| 19 | Eleonora Patuzzo | a 4'25" | Italia | Safi - Pasta Zara [Donne] |
| 20 | Regina Bruins | a 4'34" | Olanda | Cervélo Test Team [Donne] |
| 21 | Loes Gunnewijk | a 6'10" | Olanda | Nederland Bloeit [Donne] |
| 22 | Edwige Pitel | a 6'10" | Francia | S.C. Michela Fanini Record Rox [Donne] |
| 23 | Inga Cilvinaite | a 6'10" | Lituania | Safi - Pasta Zara [Donne] |
| 24 | Valentina Carretta | a 6'10" | Italia | Top Girls Fassa Bortolo - Ghezzi [Donne] |
| 25 | Linda Melanie Villumsen Serup | a 6'10" | Danimarca | HTC - Columbia Women [Donne] |
| 26 | Eva Lechner | a 8'20" | Italia | Centro Sportivo Esercito [Donne] |
| 27 | Gloria Presti | a 8'57" | Italia | Top Girls Fassa Bortolo - Ghezzi [Donne] |
| 28 | Kirsten Wild | a 10'20" | Olanda | Cervélo Test Team [Donne] |
| 29 | Adrie Visser | a 10'20" | Olanda | HTC - Columbia Women [Donne] |
| 30 | Carla Swart | a 10'20" | Sudafrica | MTN [Donne] |
